I made this in Photoshop CS3, there are about 50 layer and everything is done with brush and pen tool, I added a little spider web, painted and the work was done;)
Here's the PSD SS: http://www.imagesforme.com/show.php/1123987_eyepsd.jpg
Really vivid detail on the eye, I like the black outline it makes the red stand out a lot and it works. The cobweb out the eye could be a little bolder, it gets lost a little within the image.
Awesome though. :)