I made this in Photoshop CS3, there are about 50 layer and everything is done with brush and pen tool, I added a little spider web, painted and the work was done
Here's the PSD SS: http://www.imagesforme.com/show.php/1123987_eyepsd.jpg

work:

http://t1na.deviantart.com/art/Mad-eye-163716345

c&c?